CatG4R antisense DNA is added as a detection probe for the Cas12a reaction. After the cleavage reaction of CatG4R by Cas12a, CatG4 nucleic acid is then added. CatG4 and hemin can form an activated G-quadruplex-hemin complex, which catalyses ABST 2- and H 2 O 2 to produce ABST - and turn the solution green. The mechanism of action of antisense oligonucleotide (ASO)-based therapies that are designed to suppress gene expression. The genetic information of a cell is stored in the form of a double-stranded DNA in the nucleus. 1. Make sense and antisense mex-3 RNA in vitro 2. Inject either mex-3 antisense RNA or a mixture of mex-3 sense and antisense RNA into the gonads of C. elegans 3. Incubate and subject early embryos to in situ hybridization 4. Observe embryos under the microscope. We used morpholino backbone chemistry to make steric block antisense oligos. Unmodified DNA and RNA oligos are rapidly degraded in biological systems by enzymatic cleavage of the phosphodiester bond (Supplementary Figure S3B, R = O −) and are prone to hydrolysis due to the presence of a hydroxyl group on the 2′-carbon of the ribose sugar Antisense oligonucleotides can bind to specific sequences through Watson-Crick hybridization, which probably inhibit the combination of RNA and other nucleic acids, affecting the process of translation and splicing. 2) RNA cleavage. Antisense oligonucleotides are capable of hybridizing RNA to form an RNA-DNA duplex. NBTs, such as antisense oligonucleotides, natural antisense transcript-specific oligonucleotides (AntagoNATs), small activating RNAs and microRNA blockers (antagomirs), modulate non-coding RNA Antisense transcripts are necessary for gene activation. To assess involvement of antisense transcripts in the regulation of gene expression by agRNAs, we obtained single-stranded oligonucleotides Oligonucleotides, Antisense. DNA. We recently reported the antisense properties of a DNA/RNA heteroduplex oligonucleotide consisting of a phosphorothioate DNA-gapmer antisense oligonucleotide (ASO) strand and its complementary phosphodiester RNA/phosphorothioate 2'-O-methyl RNA strand. When α-tocopherol was conjugated with the compl …. OXpEnEL.